Giselle M. Knudsen
Position
Assistant Research Scientist
Education
| Ph.D. | Chemistry and Chemical Biology | 2003 | University of California, San Francisco |
| B.S. | Chemistry and Biochemistry | 1997 | University of Michigan, Ann Arbor |
Work History
| 2011-Present: | Assistant Research Scientist, Department of Pharmaceutical Chemistry, University of California, San Francisco |
| 2008-2011: | Research Scientist, Sandler Center for Drug Discovery, University of California, San Francisco |
| 2006-2008: | Postdoctoral Fellow, Bindley Bioscience Center, Purdue University, IN |
| 2004-2006: | Postdoctoral Trainee, Purdue University, IN |
| 2004 | Postdoctoral Researcher, Department of Pathology, University of California, San Francisco |
Research Interests
My training is in chemical biology, pharmacology, and enzymology. My scientific interests are focused on characterizing host-pathogen protein-protein interactions as a way to reveal mechanisms of host invasion, immune evasion, and pathogen replication. Target disease areas include picornaviral infections, in collaboration with the DeRisi laboratory, and parasitic diseases including schistosomiasis, trypanosomiasis and leishmaniasis in collaboration with the Sandler Center for Drug Discovery. Within the MS Facility, I have also joined the core OGlcNAc-signaling team who are interested in understanding the significance of OGlcNAc protein modifications in the context of complex disease such as diabetes and Alzheimer's disease.
Select Publications
Knudsen, G.M., Chalkley, R.J., The Effect of Using an Inappropriate Protein Database for Proteomic Data Analysis, PLoS One 6(6), e20873 (2011). [Pubmed]
Swenerton, R.K., Knudsen, G.M., Sajid, M., Kelly, B.L., & J.H. McKerrow, Leishmania subtilisin is a maturase for the trypanothione reductase system and contributes to disease pathology, J Biol Chem. 285 (41), 31120–31129 (2010). [Pubmed]
Knudsen, G.M., Davis, B., Loethen, Y.L., Deb, S.K., Gudihal, R., Perera, P., Ben-Amotz, D., & V. Jo Davisson, Quantitative 2D-Gel Analysis of Isotope Encoded Rhodamine 6G-Labeled Protein Isoforms Using SERRS Detection, Bioconjugate Chem. 19 (11), 2212–2220 (2008). [Pubmed]
Deb, S.K., Davis, B., Knudsen, G.M., Gudihal, R., Ben-Amotz, D., & V. Jo Davisson Detection and relative quantification of proteins by SERRS using isotopic labels, J. Amer. Chem. Soc. 130(30), 9624-9625 (2008). [Pubmed]
Loethen, Y.L., Knudsen, G.M., Davis, B., Gudihal, R., Davisson, V.J., & D. Ben-Amotz, Protein Quantitation in 2-D Gels using Fluorescence with Water Raman as an Internal Standard, J. Proteome Res. 7(3), 1341-1345 (2007). [Pubmed]
Dvorak, J., Mashiyama, S.T., Braschi, S., Sajid, M., Knudsen, G.M., Hansell, E., Lim, K-C, Hsieh, I., Bahgat, M., Mackenzie, B., Medzihradszky, K.F., Babbitt, P.C., Caffrey, C.R., and McKerrow, J.H., Differential use of protease families for invasion by schistosome cercariae, Biochemie 90(2), 345-58 (2008). [Pubmed]
Knudsen, G.M., Medzihradszky, K.F., Lim, K.C., McKerrow, J.H. Proteomic analysis of Schistosoma mansoni cercarial secretions, Mol. Cell Proteomics 4(12), 1862-75 (2005) . [Pubmed]
Ghiladi, R.A., Knudsen, G.M., Medzihradszky, K.F., Ortiz de Montellano, P.R., The Met-Tyr-Trp crosslink in mycobacterium tuberculosis catalase-peroxidase (KatG): Autocatalytic formation and effect on enzyme catalysis and spectroscopic properties, J. Biol. Chem. 280, 22651-22663 (2005). [Pubmed]
Knudsen, G. M., Nishida, C. R., Mooney, S. D., and P. R. Ortiz de Montellano, Nitric-oxide synthase (NOS) reductase domain models suggest a new control element in endothelial NOS that attenuates calmodulin-dependent activity, J. Biol. Chem. 278 (34), 3814-24 (2003). [Pubmed]
Rodriguez-Crespo, I., Knudsen, G. M., Nishida, C. R., and P. R. Ortiz de Montellano, Mutation of the Five conserved Histidines in the Endothelial Nitric-oxide Synthase Hemoprotein Domain: No Evidence for a Non-heme Metal Requirement for Catalysis J. Biol. Chem. 274 (31), 21617-21624 (1999). [Pubmed]
Nishida, C. R., Knudsen, G., Straub, W., and P. R. Ortiz de Montellano, Electron Supply and Catalytic Oxidation of Nitrogen by Cytochrome P450 and Nitric Oxide Synthase, Drug Metab. Rev. 34 (3) 479-501 (2002). [Pubmed]
Koshkin, A., Knudsen, G. M., and Ortiz de Montellano, P. R. Intermolecular Interactions in the AhpC/AhpD Antioxidant Defense System of Mycobacterium tuberculosis, Arch. Biochem. Biophys., 427(1), 41-47 (2004). [Pubmed]