Instrumentation

Mass Spectrometers

Thermo Scientific Q Exactive Plus Orbitrap - nanospray infusion or LC/MS or LC/MSMS of peptides and proteins with HCD
Thermo Scientific Exactive Plus EMR Orbitrap - nanospray infusion or LC/MS of proteins or native protein complexes
Thermo Scientific LTQ-Orbitrap - nanospray infusion or LC/MS or LC/MSMS of peptides and proteins with CID or ETD
Thermo Scientific LTQ-Orbitrap Velos - nanospray infusion, LC/MS or LC/MSMS with C-trap, HCD or ETD
AB Sciex/ABI QSTAR Elite Q-TOF - LC/MSMS of peptide digests with iTRAQ/SILAC quantitation
AB Sciex/ABI QSTAR XL Q-TOF - LC/MS analysis of proteins and peptides
AB Sciex/ABI QSTAR Pulsar Q-TOF - LC/MS analysis of proteins and peptides
AB Sciex QTRAP 5500 - LC/MSMS analysis of peptides with MRM
*PE-Biosystems Voyager Elite STR MALDI-TOF - linear or reflectron MS of biomolecules

* open for training for outside users within UCSF

Off-line Chromatography

Amersham Biosciences Akta HPLC system - Large scale sample preparation using ion exchange/size exclusion/affinity/hydrophilic interaction/reversed phase chromaography
Beckman Gold HPLC system - Reversed phase sample preparation
Ultimate HPLC system with Probot (DionexLC Packings) - Reversed phase sample separation for LC-MALDI

On-line nanoLC-MS

Waters NanoAcuity LC system
Famos autosampler/Ultimate HPLC system(Dionex/LC Packings)
Spark autosampler/Eksigent nanopump
Spark autosampler/Eldex syringe pump
Agilent Technologies 1100 series nanoHPLC system


National Institute of General Medical Sciences Adelson Medical Research Foundation