Posters

This Symposium has Concluded

Poster Session A

A.01 Global Quantitative Analysis of the IR Response of p53K317R Knockin Mouse Thymocytes.
E. Appella*, L. M. Miller Jenkins, S. J. Mazur, M. Rossi, and Y. Xu
A.02 withdrawn
A.03 Differential Recovery of Peptides from Sample Tubes and the Reproducibility of Quantitative Proteomic Data.
S. Bark* and V. Hook
A.04 Identification of Protein Complexes Associated to p8, a Protein Related to Tumor Progression.
M.P. Valacco*, C. Varone, J.L. Iovanna, S. Moreno, and A.L. Burlingame
A.05 Quantification of Secreted Proteins from Mycobacterium tuberculosis Identifies Alternative Secretion Pathways and Elucidases Molecular Mechanisms of Secretion.
M. Champion*, P. DiGiuseppe, and J. Cox
A.06 Methodological Refinements in iTRAQTM Reagent-Based Tagless Strategy of Identification and Purification of Soluble Protein Complexes in Bacteria.
M. Dong, H. Liu, S. Allen, S. Hall, S. Fisher, T. Hazen, J. Geller, M. Singer, L. Yang, J. Jin, M. Biggin, and H.E. Witkowska*
A.07 Spectral Clustering for Increasing MSMS-based Peptide Identifications and Identifying Unknown Modifications.
J. Falkner*, B. Searle, and P. Andrews
A.08 withdrawn
A.09 Distinct Assembly of Proteasome Complexes Contributes to Proteome Heterogeneity and Proteolytic Function in Murine Heart and Liver.
A. V. Gomes*, G. Young, Y. Wang, C. Zong, O. Drews, and P. Ping
A.10 Characterization of Intact Prion Proteins.
S. Guan*, F. Li, A. Serban, S. B. Prusiner, and A. L. Burlingame
A.11 De novo Sequencing of Antibody Variable Regions Using Methods for the Capture or Detection of Peptides Containing Cysteine.
L. Phu*, V. Pham, J. Lill, and D. Arnott
A.12 Following the Fate of the Radiolabel from [32P]PEP to Protein Targets Using a Combination of Chemical and Proteomics Approaches.
M. Jeyasingham*, J. Crowley, and G. Carlson
A.13 Protein Dynamics and Assembly of dsRNA Bacteriophage by Hydrogen/Deuterium Exchange Detected by Mass Spectrometry.
B. Suchanova*, V. Manole, S. Kang, L. Happonen, A. Ora, P. Prevelige, S. Butcher, and R. Tuma
A.14 Microwave-Assisted Nonspecific Proteolytic Digestion and Controlled Methylation for Glycomics Application.
J. Li*, X. Liu, and K. Chan
A.15 Characterization OF GSK3 Dependent Circadian Phosphoproteome.
K. Kaasik*, J. Allen, K. Shokat, L.J. Ptácek and Y-H Fu
A.16 Fast Detection and Quantification of Legionella SPP. and Legionella pneumophila.
X. Pennanec*, A. Dufour , C. Charrêteur, D. Haras, and K. Réhel
A.17 Proteomic Identification of the Cerebral Cavernous Malformation Signaling Complex.
T. Hilder*, M. Malone, S. Bencharit, J. Colicelli, T. Haystead, G. Johnson, and C. Wu
A.18 Analysis of the Dephosphorylation Kinetics of β-Casein by Alkaline Phosphatase Using a Label-free Quantitation Method.
J. Oses-Prieto*, S. Guan, D. A. Maltby, and A. L. Burlingame
A.19 Sample Preparation for Structure Characterization of Novel Antibiotics Produced by Bacillus Strains.
J.-M. Schmitter*, P. Bressollier, B. Verneuil, A. Ballade, and M. Urdaci
A.20 Quantitative Analysis of Rapamycin Effects in Yeast.
M. Fournier*, N. Pavelka, M. Sardiu, K. Zueckert-Gaudenz, C. Seidel, S. Swanson, L. Florens, and M. Washburn
A.21 Candidate Biomarker Indentification in the Time Dependent Progression of a Mouse Model of Lung Fibrosis.
K. Williams*, C. Lombardo, K. Blese, L. Wong, G. Hampton, and C. Hunter
A.22 Targeted Quantitative MRM Analysis for Validation of Changes in hESCs During Noggin and BMP4 Induced Differentiation.
A. Yocum*, C. Hunter, K.S. O’Shea, and P. Andrews
A.23 Associating Partners Modulate 20S Proteasome Functions in Mammalian Tissues.
G. Young*, A. Gomes, C. Zong, and P. Ping
A.24 The Mammalian 20S Proteasomes are Methylated on Arginine and Lysine Residues.
G. Young*, A. Gomes, and P. Ping
A.25 Erythromycin B – an old drug for the new millennium?.
M. Mordi, V. Boote, P. Bhadra, G. Morris, and J. Barber*
A.26 Phosphoproteome Profiling Approaches for Comprehensive Monitoring of Cell Signaling Events in Stimulated Macrophages.
M. Marcantonio, M. Trost, M. Courcelles, M. Desjardins, and P. Thibault*
A.27 Affinity Tagged Reductive Amination: A Novel Chemical Cross-linking Strategy.
M.J. Trnka* and A.L. Burlingame
A.28 add Unraveling the Correlation of Post Translational Modifications on Histones to Apoptosis in Yeast Using Top-Down Mass Spectrometry.
L. Jiang*, M.V. Lee, C.A. Mizzen, and N.L. Kelleher
A.29 add Utility of an Absolute Quantitation Method for Optimizing On-Column Protein Load in Qualitative and Quantitative Proteomics.
K. Compson*, C.Dorschel, J.P.C. Vissers, S.J. Geromanos, K.Panchalingam, C.Utzat, and J.L. Sherley
A.30 add Identification of Potential microRNA target genes in C. elegans by Multiple Reaction Monitoring (MRM).
M. Jovanovic*, L. Reiter, V. Lange-Link, P. Picotti, S. Schrimpf, R. Aebersold, and M.O. Hengartner
A.31 add Site-specific O-GlcNAc Mapping and Quantitation: A Tool for Monitoring O-GlcNAc Dynamics and Potentials for Disease Biomarker Discovery.
Z. Wang*, K. Park, F. Comer, and G.W. Hart

Poster Session B

B.01 Comparative Expression Analysis Between Proteomic and Transcriptomic Datasets Provides Novel Insights Into TOR Signaling Regulation in Yeast.
S. Bandhakavi*, H. Xie, D. Kim, and T. Griffin
B.02 Newly Configured Intact Protein Based Analysis System (IPAS) and Its Application in Plasma Proteomics Sample Handling.
F. Xiang*, H. Wang, W. Bankert, P. Winkler, and S. Hanash
B.03 The Trials and Tribulations of Producing Manuscripts Compliant with Journal Publication Guidelines.
R. J. Chalkley* and K. F. Medzihradszky
B.04 Identification of the Protein Biomarker Sequences of Non-Genomically Sequenced Bacteria by Composite Sequence Proteomic Analysis.
C. Fagerquist*
B.05 Inactivation of Rat Liver Mitochondrial Aspartate Aminotransferase by a Halogenated Cysteine S-conjugate.
M. Villar*, Z. Niatsetskaya, A. Cooper, and A. Artigues
B.06 The LC ESI MSMS and LC MALDI MSMS Analyses of Hostpathogen Interaction: Qualitative and Quantitative Analysis of Proteins During Francisella tularensis Infection in Mouse Macrophages and in Laser Capture Microdissected Organs.
M. Hubalek*, M. Link, Z. Krocová, M. Kroca, J. Pejchal, J. Österreicher, A. Macela, and J. Stulik
B.07 Altered Proteome Biology of Cardiac Mitochondria Under Stress Conditions.
J. Zhang, D. Liem, M. Mueller, X. Li*, Y. Wang, N. Deng, T. M. Vondriska, P. Korge, W. R. MacLellan, J. N. Weiss, R. Apweiler, and P. Ping
B.08 Linking Proteolytic Activities to Mitochondrial Function: An Analysis of Cardiac Mitochondrial Degradome.
J. Zhang, X. Li*, J. N. Weiss, P. Korge, and P. Ping
B.09 Characterization of p53 post-Translational Modifications in Cos Cells by Mass Spectrometry.
A. Joubel*, K. F. Medzihradszky, R. J. Chalkley, H. Hondermarck, and A. L. Burlingame
B.10 Integrated Systems Biology Study of Factors Imfluencing Amorphadiene Production from Metabolically Engineered Escherichia coli.
F. Pingitore*, C. Petzold, F. Nowroozi, L. Kizer, A. Redding, R. Dahl, W. Shui, D. Garcia, and J. Keasling
B.11 Intelligent MS/MS Acquisition Results in Higher Sequence Coverage and More Confident Protein IDs from Complex Samples.
J. Meza*
B.12 Separation of Stable Isotopelabeled Unfolded Proteins Before MALDI MS Analysis Enables Quantification of a Wide Range of Serum Proteins.
W.-L. Liao* and I. Turko
B.13 Protein Interaction Partners of Human 1433 Protein Isoforms.
J. McRedmond*, L. Sewell, M. Sullivan, R. Edwards, and G. Cagney
B.14 Kolmogorovsmirnov Based Scores for Protein Identification Using Peptide Mass Fingerprinting.
R. Jain* and M. Wagner
B.15 Structural Analysis of Components of the Scorpion Venom Vaejovis mexicanus smithi.
S. P. Salas-Castillo, K. F. Medzihradszky*, C. F. Batista, L. Possani, S. Mahrus, J. Wells, and A. L. Burlingame
B.16 Quantitative Proteomics Using Lanthanide Tags (MeCAT).
M. Linscheid*, R. Ahrends, S. Pieper, A. Kühn, C. Scheler, and H. Schlüter
B.17 Microwave-Assisted Techniques for HighThroughput Protein Characterization.
W. Sandoval*, V. Pham, D. Arnott, and J. Lill
B.18 Increasing the Depth to which Complex Proteomes Can Be Penetrated by MuDPIT: Identifying Chromatographic, Mass Spectrometric and Computational Improvements.
C. Slaughter*, A. High, C. Galea, P. Thomas, A. Mishra, J. Gebler, M. Zhou, M. Stine, D. Finkelstein, J. Obenauer, P. Doherty, and R. Kriwacki
B.19 Proteome Analysis of Oxidative Stress Response to Cumene Hydroperoxide in Saccharomyces cerevisiae.
L. Tuli*, A. Martins, W. Sha, P. Mendes, and V. Shulaev
B.20 Characterization of Cardiac Mitochondrial Subpopulations under Physiological Conditions Following Free Flow Electrophoresis.
O. Drews*, J. Zhang, A.-S. Lee, D. Liem, X. Li, and P. Ping
B.21 Probabilities, Expectation Values, and Decoy Database Searching: Building a Unified Framework for Statistical Data Validation in Shotgun Proteomics.
A. Nesvizhskii* and H. Choi
B.22 Automating an Integrated Protein Identification and Multiplexed Quantitation Workflow that Employs an Isobaric Mass Tagging Strategy.
C. Parman*, H. Javali, S. Lindberg, and B. Xie
B.23 Quantitative Tissue Proteomics and Biofluid Metabolomics of Cholangiocarcinoma.
Y. Wang*, A. Sharif, S. Taylor-Robinson, and W. Griffiths
B.24 Subtle Modification Isotope Ratio Proteomics: Manipulation of 15N/14N Ratio in Whole Plants Using Hydroponics.
A. Laganowsky, J. Nishio, S. Bassilian, P. Souda, J. Katz, K. Faull, and J. Whitelegge*
B.25 Identification of Proteolytic Processing Sites in Human Serum by Specific, Enzymatic Labeling of N-termini.
P. Wildes* and J.A. Wells
B.26 Profiling Protein Expression in Primary Tissues Using New Informatics Tools for Label-Free Quantitative Proteomics.
P. Cutillas* and B. Vanhaesebroeck
B.27 Direct Measurement of Fluorogenic Substrate in Crude Enzyme Membrane by LC/MS.
F. Gharahdaghi*, M. Stein, M. Bock, C. Scott, and J. Arriza
B.28 Phosphorylation Specific MS/MS Scoring.
S. Payne*, M. Yau, M. Smolka, H. Zhou, and V. Bafna
B.29 add Using a Combination of Hydrophilic Interaction Chromatography and Titanium Dioxide to Isolate Phosphorylated Peptides.
A.J. Ytterberg*, R.R. Ogorzalek Loo, P. Boontheung, J. Wohlshlegel, and J.A. Loo
B.30 add Top Down Protein Sequencing Using an Ion Mobility / Time-of-Flight Mass Spectrometer.
J. Langridge*, T. McKenna, C. Hughs, M. Allen, and M. Baumert
B.31 add Revealing Altered Epigenetic Marks in Mecp2 Mutant Mouse Brain.
F. Chu*, S. Guan, F. Li, B. Panning, and A. Burlingame

Poster Session C

C.01 Purification and Proteomic Mapping of Murine Liver 19S Proteasome Complexes.
D. Wang*, M.-C. Koag, C. Zong, X. Li, A. Gomes, and P. Ping
C.02 Complementary Fragmentations of Peptides from Lys-C and Lys-N Digests.
R. V. Talroze, J. J. Allen*, S. Guan, K. M. Shokat, and A. L. Burlingame
C.03 Construction of a Peptide Database and Its Applications for LCMS based Proteomics.
B. Feild*, Y. Kim, W. FitzHugh, J. Heidbrink, J. Duff, S. Ruben, and T. He
C.04 Seasonal and Stage-Specific Protein Expression in Liver of Golden-Mantled Ground Squirrel, a Large-Scale Quantitative Analysis.
E. Epperson*, J. Rose, and S. Martin
C.05 Protein N-Myristoylation and Prenylation in an Insect Cell-Free Protein Synthesis System and Their Identification by Mass Spectrometry.
T. Suzuki*, K. Moriya, M. Ito, T. Ezure, M. Shikata, E. Ando, T. Utsumi, S. Tsunasawa, and O. Nishimura
C.06 Protein Sulfation in Drosophila Kc Cells: Which Amino Acids and Which Proteins?.
B. Gerrits*, C. Panse, B. Bodenmiller, R. Aebersold, and R. Schlapbach
C.07 Dissecting the Role of 24SHydroxycholesterol in Cholesterol Homeostasis in Neurons.
W. Griffiths*, Y. Wang, S. Muneton, J. Sjovall, and J. Jovanovic
C.08 Combinations of Post-Translational Modifications of Human Histone H3 Revealed by Tandem FTICR/ECD Mass Spectrometry.
F. Li*, R. Talroze, F. Chu, S. Guan, and A. L. Burlingame
C.09 Proteomics of Chondrosarcoma: Identification of Key-Proteins.
J. Magdalou*, F. Lionneton, P. Netter, J.-M. Vignaud, F. Sirveaux, D. Mainard, and J.-B. Vincourt
C.10 Neuroproteomics of Chromaffin Secretory Vesicles Reveals Protein Systems Utilized for Peptide Neurotransmitter Production and Secretion.
J. Wegrzyn*, S. Bark, and V. Hook
C.11 Bioinformatic Determination of Protein Isoform Representation of Tryptic Peptides from Mass Spectrometry Analysis: Designation of RAB Isoforms in Secretory Vesicles.
J. Wegrzyn* and V. Hook
C.12 Comparison of In-House and Commercial Software for Analysis of MALDI-TOF-MS Spectra of Whole Cell Lysates for Bacterial Identification.
R. Mandrell*, B. Garbus, and L. Harden
C.13 Apolipoprotein L I Mediates IFN-γ Stimulation of Normal Human Bronchial Epithelial Cells: A Proteomics Approach.
W. Liao*, K. Koo, and WS. F. Wong
C.14 Targeted Mass Spectrometric Strategy for Global Mapping of Ubiquitination on Proteins.
S. Mollah*, D. Arnott, Q. Phung, I. Wertz, V. Dixit, N. Kayagaki, and J. Lill
C.15 Phosphoprotein Identification and Phosphorylation Site Assignment Using Different Mass Spectrometers and Search Engines.
P. Ruperez*, J. Biarc, J. Trinidad, S. Guan, and A. L. Burlingame
C.16 Detection of Intrasubunit Crosslinking Interactions in the Regulatory Beta Subunit of Phosphorylase Kinase Suggest a Possible Flipflop Mechanism of Activation by Phosphorylation.
O. Nadeau*, A. Artigues, J. Sage, M. T. Villar, and G. M. Carlson
C.17 Statistical Similarities Between Transcriptomics and Quantitative Shotgun Proteomics Data.
N. Pavelka*, S. Swanson, M. Fournier, M. Pelizzola, P. Ricciardi-Castagnoli, L. Florens, and M. Washburn
C.18 Huntington’s Disease Protein Contributes to RNAi Through Association with Argonaute and Pbodies.
J. Savas*, A. Makusky, S. Markey, and N. Tanese
C.19 Proteomic Analysis of Aurora Kinases: Mitosis and the RNAi Machinery.
A. Izrael-Tomasevic*, M. Brajenovic, A. Cochran, and D. Arnott
C.20 Global Profiling of Proteolysis in Apoptosis.
S. Mahrus* and J.A. Wells
C.21 Methods for Utilizing MS3 Data in Peptide Identification Strategies.
P. Ulintz*, B. Bodenmiller, A. Yocum, R. Aebersold, A. Nesvizhskii, and P. Andrews
C.22 Liver Histidine Kinase Activity: Mass Spectrometric Detection of Histone H4 Histidine Phosphorylation.
P. Besant*, A. Abzalov, X.-L. Zu, and P. Attwood
C.23 Identification of the Missing Components of a Receptor Kinase-Mediated Steroid Signal Transduction Pathway Using Quantitative Proteomic Profiling.
W. Tang, Z. Deng, J. Oses-Prieto, X. Zhang, N. Suzuki, S. Guan, R. Chalkley, A. Burlingame, and Z.-Y. Wang*
C.24 A Novel Stable Isotope Labeling Approach for Quantitative Proteomics.
H. Zhu*, J. Zhai, Z. Huang, X. Liu
C.25 Phosphorylation Dictates Proteolytic Function of Mammalian 20S Proteasome Complexes: Insights Gained Via Phosphoproteome Analysis.
H. Lu, C. Zong*, Y. Wang, O. Drews, X. Li, A. Gomes, and P. Ping
C.26 Proteome Profiling Using an Integrated Topdown and Bottomup Strategy.
S. Wu*, N. Lourette, N. Tolic, R. Zhao, N. Manes, R. Estep, S. Wong, J. Adkins, R. Smith, and P.-T. Ljiljana
C.27 add Quantification of Protein and Phosphorylation Expression at the Synapse.
J. Trinidad*, A.Thalhammer, C.G. Specht, A. Lynn, P. Baker, R. Schoepfer, and A. Burlingame
C.28 add Slippery when Translated: Recoded and Miscoded Proteins Tax Modern Proteomic Methods.
R. Loo*, Y. Yang, H. Mouttaki, R. Gunsalus, M. McInerney, and J. Loo
C.29 add A Complementary Approach for Genome Annotation in Caenorhabditis elegans.
L. Reiter*, S. Schrimpf, M. Jovanovic, A.G. Fraser, L.W. Hillier, M. Claassen, C. Panse, O. Rinner, M. Müller, R. Aebersold, and M.O. Hengartner
C.30 add 2D-Gel Based Proteomic Analysis of Mesenteric Lymph Following Hemorrhagic Shock and Resuscitation.
A. Zurawel, J.R. Jordan, J.S. Redzic, S.S. Damle, E.E. Moore, A. Banerjee, and K. Hansen*

National Institute of General Medical SciencesAdelson Medical Research Foundation