Giselle Knudsen

Giselle M. Knudsen


Assistant Adjunct Professor



Ph.D. Chemistry and Chemical Biology 2003 University of California, San Francisco
B.S. Chemistry and Biochemistry 1997 University of Michigan, Ann Arbor

Work History

2013-present: Assistant Adjunct Professor, Department of Pharmaceutical Chemistry, University of California, San Francisco
2013-present: Associate Research Scientist, Department of Pharmaceutical Chemistry, University of California, San Francisco
2011-2012: Assistant Research Scientist, Department of Pharmaceutical Chemistry, University of California, San Francisco
2008-2011: Research Scientist, Sandler Center for Drug Discovery, University of California, San Francisco
2006-2008: Postdoctoral Fellow, Bindley Bioscience Center, Purdue University, IN
2004-2006: Postdoctoral Trainee, Purdue University, IN
2004 Postdoctoral Researcher, Department of Pathology, University of California, San Francisco

Research Interests

My training is in chemical biology, pharmacology, and enzymology. My scientific interests are focused on characterizing host-pathogen protein-protein interactions as a way to reveal mechanisms of host invasion, immune evasion, and pathogen replication. Target disease areas include picornaviral infections, in collaboration with the DeRisi laboratory, and parasitic diseases including schistosomiasis, trypanosomiasis and leishmaniasis. Within the MS Facility, I have also joined the core OGlcNAc-signaling team who are interested in understanding the significance of OGlcNAc protein modifications in the context of complex disease such as Alzheimer's disease.

Select Publications

Morris JH, Knudsen GM, Verschueren E, Johnson J, Cimermancic P, Greninger AL, Pico AR. Affinity Purification-Mass Spectrometry and Network Analysis for Understanding Protein-Protein Interactions. Nat Protoc, 9(11), 2539-54.(2014) [Pubmed]

Greninger AL, Knudsen GM, Betegon M, Burlingame AL, DeRisi JL. ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding. MBio, 4(2),e00098-13. (2013) PMC3622926. [Pubmed]

O’Donoghue AJ, Jin Y, Knudsen GM, Perera NC, Jenne DE, Murphy JE, Craik CS,Hermiston T. Global Substrate profiling of proteases in human Neutrophil Extracellular Traps reveals consensus motif predominantly contributed by Elastase. PLoS One, 8(9), e75141. (2013) [Pubmed]

Sanchez L, Knudsen G, Hartmann C, DeMuylder G, Mascuch S, Mackey Z, Gerwick L, Clayton C, McKerrow J, Linington R. Examination of the Mode of Action of the Almiramide Family of Natural Products Against the Kinetoplastid Parasite Trypanosoma brucei. J. Natural Products, 76(4), 630-41. (2013) [Pubmed]

Greninger AL, Knudsen GM, Betegon M, Burlingame AL, DeRisi JL. The 3A protein from multiple picornaviruses utilizes the Golgi Adaptor Protein ACBD3 to Recruit PI4KIII_ J. Virology, 86(7), 3605–168.(2012) [Pubmed]

O’Donoghue A, Eroy-Reveles A, Knudsen GM, Ingram J, Zhou M, Anderson M, Qu G, Maltby D, McKerrow J, Burlingame AL, Craik CS. Multiplexed Profiling of Protease Specificity Using a Physico-chemically Diverse Synthetic Peptide Library and Mass Spectrometric Detection Nature Methods DOI 10.1038/NMETH.2182. (2012) [Pubmed]

Sojka D, Franta Z, Frantová H, Bartosová P, Horn M, Váchová J, O’Donoghue AJ, Eroy-Reveles AA, Craik CS, Knudsen G.M., Caffrey CR, McKerrow JH, Mares M, Kopácek P Characterization of gut-associated cathepsin D hemoglobinase from tick Ixodes ricinus (IrCD1) J Biol Chem. 287(25), 21152–63. (2012) [Pubmed]

Davisson VJ, Deb SK, Knudsen GM, Xin M. Reagents for Biomolecular Labeling, Detection and Quantification Employing Raman Spectroscopy. US Patent No. 8,153,827 B2. (2012)

Ingram J, Knudsen GM, Lim KC, Hansell E, Sakanari J, McKerrow JH. Proteomic Analysis of Human Skin Treated with Schistosome Larval Proteases Reveals Distinct Invasion Strategies Among Species of Blood Flukes PLoS Negl Trop Dis. 5(9), e1337. (2011) [Pubmed]

Knudsen GM, Chalkley RJ. The Effect of Using an Inappropriate Protein Database for Proteomic Data Analysis, PLoS One 6(6), e20873 (2011). [Pubmed]

Swenerton RK, Knudsen GM, Sajid M, Kelly BL, McKerrow JH. Leishmania subtilisin is a maturase for the trypanothione reductase system and contributes to disease pathology, J Biol Chem. 285 (41), 31120–31129 (2010). [Pubmed]

Knudsen GM, Davis B, Loethen YL, Deb SK, Gudihal R, Perera P, Ben-Amotz D, Davisson,VJ. Quantitative 2D-Gel Analysis of Isotope Encoded Rhodamine 6G-Labeled Protein Isoforms Using SERRS Detection, Bioconjugate Chem. 19 (11), 2212–2220 (2008). [Pubmed]

Deb SK, Davis B, Knudsen GM, Gudihal R, Ben-Amotz D, Davisson VJ. Detection and relative quantification of proteins by SERRS using isotopic labels, J. Amer. Chem. Soc. 130(30), 9624-9625 (2008). [Pubmed]

Loethen YL, Knudsen GM, Davis B, Gudihal R, Davisson VJ, Ben-Amotz D. Protein Quantitation in 2-D Gels using Fluorescence with Water Raman as an Internal Standard, J. Proteome Res. 7(3), 1341-1345 (2007). [Pubmed]

Dvorak J, Mashiyama ST, Braschi S, Sajid M, Knudsen GM, Hansell E, Lim K-C, Hsieh I, Bahgat M, Mackenzie B, Medzihradszky KF, Babbitt PC, Caffrey CR, McKerrow JH. Differential use of protease families for invasion by schistosome cercariae, Biochemie 90(2), 345-58 (2008). [Pubmed]

Knudsen, GM, Medzihradszky KF, Lim KC, McKerrow JH. Proteomic analysis of Schistosoma mansoni cercarial secretions, Mol. Cell Proteomics 4(12), 1862-75 (2005) . [Pubmed]

Ghiladi RA, Knudsen GM, Medzihradszky KF, Ortiz de Montellano PR. The Met-Tyr-Trp crosslink in mycobacterium tuberculosis catalase-peroxidase (KatG): Autocatalytic formation and effect on enzyme catalysis and spectroscopic properties, J. Biol. Chem. 280, 22651-22663 (2005). [Pubmed]

Knudsen GM, Nishida CR, Mooney SD,Ortiz de Montellano PR. Nitric-oxide synthase (NOS) reductase domain models suggest a new control element in endothelial NOS that attenuates calmodulin-dependent activity, J. Biol. Chem. 278 (34), 3814-24 (2003). [Pubmed]

Rodriguez-Crespo I, Knudsen GM, Nishida CR, Ortiz de Montellano PR. Mutation of the Five conserved Histidines in the Endothelial Nitric-oxide Synthase Hemoprotein Domain: No Evidence for a Non-heme Metal Requirement for Catalysis J. Biol. Chem. 274 (31), 21617-21624 (1999). [Pubmed]

Nishida CR, Knudsen G, Straub W, Ortiz de Montellano PR. Electron Supply and Catalytic Oxidation of Nitrogen by Cytochrome P450 and Nitric Oxide Synthase, Drug Metab. Rev. 34 (3) 479-501 (2002). [Pubmed]

Koshkin A, Knudsen GM, Ortiz de Montellano PR. Intermolecular Interactions in the AhpC/AhpD Antioxidant Defense System of Mycobacterium tuberculosis, Arch. Biochem. Biophys., 427(1), 41-47 (2004). [Pubmed]

National Institute of General Medical Sciences Adelson Medical Research Foundation