Assistant Research Biochemist
| Ph.D. | Chemistry | 2007 | University of Utah, Salt Lake City, UT |
| B.S. | Biochemistry | 2000 | Indiana University, Bloomington, IN |
| 2013 – Present: | Assistant Research Biochemist, Department of Pharmaceutical Chemistry, University of California, San Francisco |
| 2008 – 2013: | Postdoctoral Fellow, Department of Pharmaceutical Chemistry, University of California, San Francisco |
| 2002 – 2007: | Graduate Research Assistant, Department of Chemistry, Univversity of Utah, Salt Lake City |
| 2000 – 2001: | Bioanalytical Chemist, Pharmaceutical Product Development, Eli Lilly & Co., Indianapolis, IN |
Choy JW, Bryant C, Calvet CM, Doyle PS, Gunatilleke SS , Leung SSF, Ang KH, Chen S, Gut J, Oses-Prieto JA, Johnston JB, Arkin MR, Burlingame AL, Taunton J, Jacobson MP, McKerrow JH, Podust LM, Renslo AR. Chemical-biological characterization of a cruzain inhibitor reveals a second target and a mammalian off-target, Beilstein J. Org. Chem. 9, 15–25. (2013) [Pubmed]
Gunatilleke SS, Calvet CM, Johnston JB, Chen CK, Erenburg G, Gut J, Engel JC, Ang KH, Mulvaney J, Arkin MR, McKerrow JH, Podust LM. Diverse inhibitor chemotypes targeting Trypanosoma cruzi CYP51, PLoS Negl. Trop. Dis. 6(7), e1736. (2012) [Pubmed]
Johnston JB, Singh AA, Clary AA, Chen CK, Chow S, Hayes PY, De Voss JJ, Ortiz de Montellano PR. Substrate analog studies of the ω-regiospecificity of Mycobacterium tuberculosis cholesterol metabolizing cytochrome P450 enzymes CYP124A1, CYP125A1, and CYP142A1, Bioorg. Med. Chem. 20, 4064–81. (2012) [Pubmed]
Jensen K, Johnston JB, Ortiz de Montellano PR, Moller BL. Photosystem I from plants as a bacterial cytochrome P450 surrogate electron donor: terminal hydroxylation of branched hydrocarbon chains, Biotechnol. Lett. 34, 239–45. (2011) [Pubmed]
Ouellet H, Johnston JB, Ortiz de Montellano PR. Cholesterol catabolism as a therapeutic target in Mycobacterium tuberculosis, Trends Microbiol. 19(11), 530–39. (2011) [Pubmed]
Johnston JB, Ouellet H, Podust LM, Ortiz de Montellano PR. Structural control of Cytochrome P450- catalyzed ω-hydroxylation, Arch. Biochem. Biophys. 507, 86–94. (2011) [Pubmed]
Park H, Lee I, Chun Y, Yun C, Johnston JB, Ortiz De Montellano PR, Kim D. Heterologous expression and characterization of sterol 14α-demethylase, CYP51F1 from Candida albicans, Arch. Biochem. Biophys. 509(1), 9–15. (2011) [Pubmed]
Johnston JB, Ouellet H, Ortiz de Montellano PR. Functional redundancy of steroid 26 –monooxygenase activity in Mycobacterium tuberculosis revealed by biochemical and genetic analyses, J. Biol. Chem. 285(47), 36352–60. (2010) [Pubmed]
Ouellet H, Guan S, Johnston JB, Chow ED, Kells PM, Burlingame AL, Cox JS, Podust LM, Ortiz de Montellano PR. Mycobacterium tuberculosis CYP125A1, a steroid-27-monooxygenase that detoxifies intracellularly generated cholest-4-ene-3-one, Mol. Microbiol. 77(3), 730–42. (2010) [Pubmed]
Doyle PS, Chen C, Johnston JB, Hopkins S, Leung SF, Jacobson MP, Engel JC, McKerrow JH, Podust LM. A non-azole CYP51 inhibitor cures Chagas disease in a mouse model of acute infection, Antimicrob. Agents Ch. 54(6), 2480–8. (2010) [Pubmed]
Ouellet H, Johnston JB, Ortiz de Montellano PR. The Mycobacterium tuberculosis cytochrome P450 system, Arch. Biochem. Biophys. 493(1), 82 –95. (2010) [Pubmed]
Johnston JB, Kells PM, Podust LM, Ortiz de Montellano PR. Biochemical and structural characterization of CYP124: a methyl-branched lipid ω-hydroxylase from Mycobacterium tuberculosis, Proc. Nat. Acad. Sci. U.S.A. 106(49), 20687–92. (2009) [Pubmed]
Rothman SC, Johnston JB, Lee S, Walker JR, Poulter CD. Type II isopentenyl diphosphate isomerase: irreversible inactivation by covalent modification of flavin, J. Am. Chem. Soc. 130(14), 4906–13. (2008) [Pubmed]
Johnston JB, Rothman SC, Walker JR, Poulter CD. Type-2 isopentenyl diphosphate isomerase. Mechanistic studies with cyclopropyl and epoxy analogues. J. Am. Chem. Soc. 129(25), 7740–1. (2007) [Pubmed]
