Jonathan Trinidad
Position
Assistant Adjunct Professor
Education
| Ph.D. | Neuroscience | 2002 | Harvard Medical School, Boston, MA |
| B.Sc. | Chemistry | 1996 | The College of William and Mary, Williamsburg, VA |
Work History
| 2005-Present: | Assistant Adjunct Professor, Dept. of Pharmaceutical Chemistry, UCSF, CA |
| 2003-2005: | Postdoctoral Fellow, Mass Spectrometry Facility, UCSF, CA |
Research Interests
I am interested in understanding the functioning of biological systems at the molecular level. My work primarily deals with synapses in the central nervous system. Synapses are sites of neuron-neuron communication where chemicals known as neurotransmitters are released by one neuron and detected by the other. This detection causes electrical excitation of the downstream neuron. A highly organized protein machine is responsible for detecting neurotransmitters and initiating electrical excitation. In addition to detecting neurotransmitter, the synaptic protein machinery can change its composition to alter the strength of subsequent neuron-neuron communication. These changes are encoded by differential protein expression as well as changes in levels of the protein post-translational modifications. We are interested in using mass spectrometry to quantify proteins and posttranslational modifications at the synapse and use this data to gain a deeper understanding into synaptic physiology.
Select Publications
David, D.C., Ollikainen, N., Trinidad, J.C., Cary, M.P., Burlingame, A.L., Kenyon, C., Widespread protein aggregation as an inherent part of aging in C. elegans, PLoS Biology 8(8), e1000450 (2010). [Pubmed]
Beltrao, P., Trinidad, J.C., Fiedler, D., Toguev, A., Lim, W.A., Shokat, K.M., Burlingame, A.L., Krogan, N.J., Evolution of Phosphoregulation: Comparison of Phosphorylation Patterns across Yeast Species, PLoS Biology 7(6), e1000134 (2009). [Pubmed]
Fernandez, F., Trinidad, J.C., Blank, M., Feng, D.D., Burlingame, A.L., Garner, C.C., Normal protein composition of synapses in Ts65Dn mice: a mouse model of Down syndrome, Journal of Neurochemistry 110(1) 157-69 (2009). [Pubmed]
Mahrus, S., Trinidad, J.C., Barkan, D.T., Sali, A., Burlingame, A.L., Wells, J.A., Global Sequencing of Proteolytic Cleavage Sites in Apoptosis by Specific Labeling of Protein N Termini, Cell 134(4), 868-876 (2008). [Pubmed]
Trinidad, J.C., Thalhammer, A., Specht, C.G., Lynn, A.J., Baker, P.R., Schoepfer, R., and Burlingame, A.L., Quantitative Analysis of Synaptic Phosphorylation and Protein Expression, Molecullar & Cellular Proteomics 7(4), 684-696 (2008). [Pubmed]
Olsen, O., Funke, L., Long, J.F., Fukata, M., Kazuta, T., Trinidad, J.C., Moore, K.A., Misawa, H., Welling, P.A., Burlingame, A.L., Zhang, M., and Bredt, D.S., Renal defects associated with improper polarization of the CRB and DLG polarity complexes in MALS-3 knockout mice, Journal of Cell Biology, 179(1), 151-164, (2007). [Pubmed]
Vosseller, K., Trinidad, J.C., Chalkley, R.J.., Specht, C.G., Thalhammer, A., Lynn, AJ., Snedecor, AO., Guan, S., Medzihradszky, K.F., Maltby, D., Schoepfer, R., and Burlingame, A.L., O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry, Molecullar & Cellular Proteomics, 5(5), 923-934 (2006). [Pubmed]
Trinidad, J.C., Thalhammer, A., Specht, C.G., Schoepfer R., and Burlingame A.L., Comprehensive Identification of Phosphorylation Sites in Postsynaptic Density Preparations, Molecullar & Cellular Proteomics, 5(5), 914-922 (2006). [Pubmed]
Trinidad, J.C., Thalhammer, A., Specht, C.G., Schoepfer R., and Burlingame A.L., Phosphorylation State of Postsynaptic Density Proteins, J Neurochem, 92, 1306-1316 (2005). [Pubmed]
Fukata, Y., Tzingounis, A.V., Trinidad, J.C., Fukata, M., Burlingame, A.L., Nicoll, R.A., and Bredt, D.S., Molecular Constituents of Neuronal AMPA Receptors, J Cell Biol, 169, 399-404 (2005). [Pubmed]
Olsen, O., Moore, K.A., Fukata, M., Kazuta, T., Trinidad, J.C., Kauer, F.W., Streuli, M., Misawa, H., Burlingame, A.L., Nicoll, R.A., and Bredt, D.S., Neurotransmitter Release Regulated by a MALS / Liprin-α Presynaptic Complex, Journal of Cell Biology, 170(7), 1127-1134, (2005). [Pubmed]