At The Source: MSF Blog

Author Archives: Mark Burlingame

Lectin Weak Affinity Chromatography (LWAC) Protocol posted to the MSF site

The lectin wheat germ agglutinin (WGA) has affinity for terminal N-acetylglucosamine (GlcNAc) and sialic acid residues. It has four binding sites, so can bind with high affinity to branched glycan structures with multiple terminal GlcNAc moieties. However, the affinity for a single GlcNAc residue, as encountered with the regulatory modification of O-GlcNAcylation, found on serines and threonines of nuclear and cytoplasmic proteins, is low. Hence, our Resource has developed and employs a protocol where WGA attached to POROS is used as a weak affinity column to enrich for O-GlcNAc-modified peptides.  You can find the protocol here:

LWAC protocol